Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN953980 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-P21-Activated Kinase 6 (PAK6) (AA 275-305), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 275-305 amino acids from the Central region of human PAK6
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 275-305,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Distinct DNA methylation profiles between adenocarcinoma and squamous cell carcinoma of human uterine cervix.
A Large-scale genetic association study of esophageal adenocarcinoma risk.
Genetic susceptibility to distinct bladder cancer subphenotypes.
Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China.
PTEN identified as important risk factor of chronic obstructive pulmonary disease.
Lee EJ, Mcclelland M, Wang Y, Long F, Choi SH, Lee JH
Oncology research 2010;18(9):401-8
Oncology research 2010;18(9):401-8
A Large-scale genetic association study of esophageal adenocarcinoma risk.
Liu CY, Wu MC, Chen F, Ter-Minassian M, Asomaning K, Zhai R, Wang Z, Su L, Heist RS, Kulke MH, Lin X, Liu G, Christiani DC
Carcinogenesis 2010 Jul;31(7):1259-63
Carcinogenesis 2010 Jul;31(7):1259-63
Genetic susceptibility to distinct bladder cancer subphenotypes.
Guey LT, García-Closas M, Murta-Nascimento C, Lloreta J, Palencia L, Kogevinas M, Rothman N, Vellalta G, Calle ML, Marenne G, Tardón A, Carrato A, García-Closas R, Serra C, Silverman DT, Chanock S, Real FX, Malats N, EPICURO/Spanish Bladder Cancer Study investigators
European urology 2010 Feb;57(2):283-92
European urology 2010 Feb;57(2):283-92
Polymorphisms in innate immunity genes and lung cancer risk in Xuanwei, China.
Shen M, Vermeulen R, Rajaraman P, Menashe I, He X, Chapman RS, Yeager M, Thomas G, Burdett L, Hutchinson A, Yuenger J, Chanock S, Lan Q
Environmental and molecular mutagenesis 2009 May;50(4):285-90
Environmental and molecular mutagenesis 2009 May;50(4):285-90
PTEN identified as important risk factor of chronic obstructive pulmonary disease.
Hosgood HD 3rd, Menashe I, He X, Chanock S, Lan Q
Respiratory medicine 2009 Dec;103(12):1866-70
Respiratory medicine 2009 Dec;103(12):1866-70
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PAK6 Antibody (Center) Cat.-No AP53154PU-N in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PAK6 Antibody (Center) Cat.-No AP53154PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with AP53154PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.