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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB1167 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Prolactin R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Prolactin R in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Prolactin R and recombinant rat Prolactin R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P16471- Isotype
- IgG
- Antibody clone number
- 250448
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The transcriptional responsiveness of LKB1 to STAT-mediated signaling is differentially modulated by prolactin in human breast cancer cells.
High expression of prolactin receptor is associated with cell survival in cervical cancer cells.
Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
Linher-Melville K, Singh G
BMC cancer 2014 Jun 9;14:415
BMC cancer 2014 Jun 9;14:415
High expression of prolactin receptor is associated with cell survival in cervical cancer cells.
Lopez-Pulido EI, Muñoz-Valle JF, Del Toro-Arreola S, Jave-Suárez LF, Bueno-Topete MR, Estrada-Chávez C, Pereira-Suárez AL
Cancer cell international 2013 Oct 22;13(1):103
Cancer cell international 2013 Oct 22;13(1):103
Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
Kanda N, Watanabe S
Endocrinology 2007 May;148(5):2317-25
Endocrinology 2007 May;148(5):2317-25
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Prolactin R Inhibition of Prolactin-dependent Cell Proliferation and Neutralization by Human Prolactin R Antibody. Recombinant Human Prolactin R Fc Chimera (Catalog # 1167-PR) inhibits Recombinant Human Prolactin (Catalog # 682-PL) induced proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human Prolactin (0.5 ng/mL) activity elicited by Recombinant Human Prolactin R Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Prolactin R Monoclonal Antibody (Catalog # MAB1167). The ND50 is typically 2.5-9 µg/mL.
