PAB20186

antibody from Abnova Corporation

Targeting: CD2AP CMS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20186

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20186, RRID:AB_10963726

Product name

CD2AP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CD2AP.

Antigen sequence

WSGTLNNKLGLFPSNFVKELEVTDDGETHEAQDDS
ETVLAGPTSPIPSLGNVSETASGSVTQPKKIRGIG
FGDIFKEGSVKLRTRTSSSETEEKKPEKPLILQSL
GPKTQSVEITKTDTEGKIKAKE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CD2AP polyclonal antibody (Cat # PAB20186) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with CD2AP polyclonal antibody (Cat # PAB20186) at 1-4 ug/mL dilution shows positivity in plasma membrane, cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with CD2AP polyclonal antibody (Cat # PAB20186) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)