Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96283 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPH3AL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KYILPLKTPG RADDPHFRPL PTEPAEREPR SSETSRIYTW ARGRVVSSDS DSDSDLSSSS LEDRLPSTGV RDRKGDKPWK ESGGSVEAPR MGFTHPPGHL SGCQSSLASG ETGTGSADPP GGPRPGLTRR APVKDTPGRA PAADAAPAGP SSCLG; Positive Samples: BT-474, 22Rv1, SW620, NCI-H460; Cellular Location: Cytoplasm, Cytoplasmic vesicle, secretory vesicle membrane
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.45 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPH3AL in extracts of various cell lines using RPH3AL Polyclonal Antibody (Product # PA5-96283) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPH3AL in paraffin-embedded human liver using RPH3AL Polyclonal Antibody (Product # PA5-96283) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPH3AL in paraffin-embedded human stomach using RPH3AL Polyclonal Antibody (Product # PA5-96283) at a dilution of 1:200.