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LearnPAB10226
antibody from Abnova Corporation
Targeting: NIPBL
DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, IDN3, Scc2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- PAB10226 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10226, RRID:AB_1679617
- Product name
- NIPBL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of NIPBL.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references NIPBL gene responsible for Cornelia de Lange syndrome, a severe developmental disorder.
NIPBL mutational analysis in 120 individuals with Cornelia de Lange syndrome and evaluation of genotype-phenotype correlations.
NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome.
Ben-Asher E, Lancet D
The Israel Medical Association journal : IMAJ 2004 Sep;6(9):571-2
The Israel Medical Association journal : IMAJ 2004 Sep;6(9):571-2
NIPBL mutational analysis in 120 individuals with Cornelia de Lange syndrome and evaluation of genotype-phenotype correlations.
Gillis LA, McCallum J, Kaur M, DeScipio C, Yaeger D, Mariani A, Kline AD, Li HH, Devoto M, Jackson LG, Krantz ID
American journal of human genetics 2004 Oct;75(4):610-23
American journal of human genetics 2004 Oct;75(4):610-23
NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome.
Tonkin ET, Wang TJ, Lisgo S, Bamshad MJ, Strachan T
Nature genetics 2004 Jun;36(6):636-41
Nature genetics 2004 Jun;36(6):636-41
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of a band ~300 KDa correspondingto NIPBL (arrowhead) in human kidney (lane1) and human heart (Lane 2) whole cell tissue extracts.Approximately 20 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:500.Use HRP conjugated Rb-a-Goat IgG [H&L] MXHu for detection.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using NIPBL polyclonal antibody (Cat # PAB10226) shows detection of bands at ~315 KDa and ~125 KDa corresponding to isoforms of NIPBL (arrow-heads) in mouse heart whole cell tissue extract.Approximately 35 ug of lysate was separated on a 4-8% gel by SDS-PAGE and transferred onto nitrocellulose.After blocking the membrane was probed with the primary antibody diluted to 1:1,500.Reaction occurredovernight at 4°C followed by washes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trade mark of LI-COR, Inc.
