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LearnCF503326
antibody from Invitrogen Antibodies
Targeting: NEK9
DKFZp434D0935, MGC16714, Nek8, NERCC, NERCC1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- CF503326 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK9 Monoclonal Antibody (OTI1H1), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1H1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (RC211326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9. Positive lysates LY409721 (100 µg) and LC409721 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK9 (RC211326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK9. Positive lysates LY409721 (100 µg) and LC409721 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 6 different cell lines by using anti-NEK9 monoclonal antibody. (1:200)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NEK9-Knockout hela cells (KO,Cat# LC810398) were separated by SDS-PAGE and immunoblotted with anti-NEK9 monoclonal antibody TA503326. Then the blotted membrane was stripped and reprobed with anti-beta-actin (TA811000) as a loading control. (1:500)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-NEK9 mouse monoclonal antibody (TA503326) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK9(RC211326).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC211326 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK9 antibody (TA503326), and then analyzed by flow cytometry.
