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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- NBP1-86624 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86624, RRID:AB_11037737
- Product name
- Rabbit Polyclonal CARS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat CARS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CARS Antibody [NBP1-86624] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CARS Antibody [NBP1-86624] - Analysis using Anti-CARS antibody NBP1-86624 (A) shows similar pattern to independent antibody NBP1-86623 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining in human cerebral cortex and skeletal muscle tissues using anti-CARS antibody. Corresponding CARS RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human cerebral cortex, pancreas, skeletal muscle and testis using Anti-CARS antibody NBP1-86624 (A) shows similar protein distribution across tissues to independent antibody NBP1-86623 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human pancreas.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CARS Antibody [NBP1-86624] - Staining of human testis.
