MA5-34763

antibody from Invitrogen Antibodies

Targeting: ILF3 DRBP76, MPHOSPH4, MPP4, MPP4110, NF110, NF110b, NF90, NF90a, NF90c, NF90ctv, NFAR-1, NFAR-2, NFAR110, NFAR90, TCP110

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34763

Provider

Invitrogen Antibodies

Product name

ILF3 Recombinant Rabbit Monoclonal Antibody (JG37-63)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: Rat heart tissue lysate, 293, A431, SH-SY-5Y, SiHa, human tonsil tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue, mouse kidney tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG37-63

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ILF3 in rat heart tissue (1) and 293 cell (2) lysate. Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ILF3 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ILF3 monoclonal antibody (Product # MA5-34763), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ILF3 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ILF3 monoclonal antibody (Product # MA5-34763), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ILF3 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with ILF3 monoclonal antibody (Product # MA5-34763), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse testis tissue. Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse kidney tissue. Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ILF3 in paraffin-embedded human tonsil tissue. Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ILF3 in paraffin-embedded human prostate cancer tissue. Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon tissue. Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ILF3 in K562 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with ILF3 monoclonal antibody (Product # MA5-34763) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.