Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 646403 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#646403, RRID:AB_2563004
- Product name
- PE anti-IRF4
- Antibody type
- Monoclonal
- Antigen
- GST fusion protein containing C-terminal of murine IRF4
- Description
- The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Yellow dye
- Antibody clone number
- IRF4.3E4
- Vial size
- 25 µg
- Concentration
- 0.2 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- PHA-stimulated (3 days) or freshly isolated human peripheral lymphocytes were stained with CD4 APC, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601), and then stained with IRF4 (clone IRF4.3E4) PE (open histogram with solid line for fresh cells, filled histogram for stimulated cells) or rat IgG2a, κ PE isotype control (dash line for fresh cell, dot line for stimulated cells). Data shown was gated on CD4+ lymphocyte population.
- Conjugate
- Yellow dye