H00003662-M05

antibody from Novus Biologicals

Targeting: IRF4 LSIRF, MUM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00003662-M05

Provider

Novus Biologicals

Proper citation

Novus Cat#H00003662-M05, RRID:AB_829232

Product name

Mouse Monoclonal IRF4 Antibody

Antibody type

Monoclonal

Description

IgG purified. IRF4 (2D1)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi or non-transfected control. Blot probed with H00003662-M05. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody (M05), clone 2D1. Lane 1: IRF4 transfected lysatE (51.8 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: IRF4 Antibody (2D1) [H00003662-M05] - Detection limit for recombinant GST tagged IRF4 is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: IRF4 Antibody (2D1) [H00003662-M05] - Analysis of monoclonal antibody to IRF4 on HeLa cell. Antibody concentration 10 ug/ml