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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Other assay [1]
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- Product number
- PA5-21144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Jurkat cell lysate.
- Concentration
- 1 mg/mL
Submitted references Alpha-1 Antitrypsin Attenuates M1 Microglia-Mediated Neuroinflammation in Retinal Degeneration.
Zhou T, Huang Z, Zhu X, Sun X, Liu Y, Cheng B, Li M, Liu Y, He C, Liu X
Frontiers in immunology 2018;9:1202
Frontiers in immunology 2018;9:1202
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat cell lysate using a IRF4 polyclonal antibody (Product # PA5-21144) at 1 µg/mL in either (A) the presence and (B) the absence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IRF4 in Ramos cell lysate with IRF4 Polyclonal Antibody (Product # PA5-21144) at 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of Jurkat cells using a IRF4 polyclonal antibody (Product # PA5-21144) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Jurkat cells using a IRF4 polyclonal antibody (Product # PA5-21144) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of IRF4 in Jurkat cells with IRF4 Polyclonal Antibody (Product # PA5-21144) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of IRF4 in Jurkat cells with IRF4 Polyclonal Antibody (Product # PA5-21144) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Alpha-1 antitrypsin (AAT) modulated IRF4/8 activation and phosphorylation of STAT1 in vivo and in vitro . (A,B) Western blot revealed that in AAT-treated retina of rd1 mice, the IRF8 expression significantly decreased while the expression of IRF4 increased slightly. Moreover, the STAT1 signaling was suppressed after AAT supplement. (C,D) In vitro , hydrogen peroxide stimulation upregulated the expression level of IRF8 and promoted phosphorylation of STAT1 in the primary cultured microglia, while AAT treatment reversed the expression trend with decreased IRF8 and STAT1 and increased IRF4. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed unpaired t -test).
