AF6286

antibody from R&D Systems

Targeting: TDRKH TDRD2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6286

Provider

R&D Systems

Product name

Human TDRKH Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human TDRKH in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9Y2W6


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human TDRKH by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Human TDRKH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6286) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TDRKH at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TDRKH in Mouse Testis. TDRKH was detected in immersion fixed frozen sections of adult mouse testis using Human TDRKH Affinity-purified Polyclonal Antibody (Catalog # AF6286) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to germ cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.