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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- MA5-34915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGP9.5 Monoclonal Antibody (B1-5-6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: A172, SHG-44, A549, SH-SY5Y, rat cerebellum tissue, human kidney tissue, human pancreas tissue, mouse brain tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B1-5-6
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PGP9.5 in A172 (1) and SHG-44 (2). Samples were incubated with PGP9.5 monoclonal antibody (Product # MA5-34915), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PGP9.5 in SH-SY-5Y cells. Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PGP9.5 monoclonal antibody (Product # MA5-34915), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGP9.5 in paraffin-embedded rat cerebellum tissue. Samples were incubated with PGP9.5 monoclonal antibody (Product # MA5-34915), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGP9.5 in paraffin-embedded mouse brain tissue. Samples were incubated with PGP9.5 monoclonal antibody (Product # MA5-34915), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGP9.5 in paraffin-embedded human pancreas tissue. Samples were incubated with PGP9.5 monoclonal antibody (Product # MA5-34915), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PGP9.5 in paraffin-embedded human kidney tissue. Samples were incubated with PGP9.5 monoclonal antibody (Product # MA5-34915), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PGP9.5 in A549 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with PGP9.5 monoclonal antibody (Product # MA5-34915) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG.
