AF3040

antibody from R&D Systems

Targeting: IL32 NK4, TAIF, TAIFb, TAIFd

Western blot (Data presented on provider website)

Antibody Data

Product number

AF3040

Provider

R&D Systems

Product name

Human IL-32 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human IL-32 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

NP_001012651


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Genetic variant in IL-32 is associated with the ex vivo cytokine production of anti-TNF treated PBMCs from rheumatoid arthritis patients.

Damen MSMA, Schraa K, Tweehuysen L, den Broeder AA, Netea MG, Popa CD, Joosten LAB
Scientific reports 2018 Sep 19;8(1):14050

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Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1.

Palstra RJ, de Crignis E, Röling MD, van Staveren T, Kan TW, van Ijcken W, Mueller YM, Katsikis PD, Mahmoudi T
Science advances 2018 Feb;4(2):e1701729

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Interleukin (IL)-32β-mediated CCAAT/enhancer-binding protein α (C/EBPα) phosphorylation by protein kinase Cδ (PKCδ) abrogates the inhibitory effect of C/EBPα on IL-10 production.

Kang JW, Park YS, Kim MS, Lee DH, Bak Y, Ham SY, Park SH, Hong JT, Yoon DY
The Journal of biological chemistry 2013 Aug 16;288(33):23650-8

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Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation.

Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashi R, Kinoshita H, Sakamoto K, Nakagawa H, Akanuma M, Yoshida H, Maeda S, Koike K
Infection and immunity 2012 Nov;80(11):3795-803

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Inhibition of IL-32 activation by α-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.

Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ
Blood 2011 Nov 3;118(18):5031-9

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Inflammation-dependent secretion and splicing of IL-32{gamma} in rheumatoid arthritis.

Heinhuis B, Koenders MI, van de Loo FA, Netea MG, van den Berg WB, Joosten LA
Proceedings of the National Academy of Sciences of the United States of America 2011 Mar 22;108(12):4962-7

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Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.

Hasegawa H, Thomas HJ, Schooley K, Born TL
Cytokine 2011 Jan;53(1):74-83

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Protection from RNA and DNA viruses by IL-32.

Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8

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Interleukin-32 expression in the pancreas.

Nishida A, Andoh A, Inatomi O, Fujiyama Y
The Journal of biological chemistry 2009 Jun 26;284(26):17868-76

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Epithelial overexpression of interleukin-32alpha in inflammatory bowel disease.

Shioya M, Nishida A, Yagi Y, Ogawa A, Tsujikawa T, Kim-Mitsuyama S, Takayanagi A, Shimizu N, Fujiyama Y, Andoh A
Clinical and experimental immunology 2007 Sep;149(3):480-6