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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10608 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10608, RRID:AB_11188911
- Product name
- BIRC5 monoclonal antibody, clone 1H5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant BIRC5.
- Isotype
- IgG
- Antibody clone number
- 1H5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against HEK293 (1) and BIRC5 (aa 1-142) -hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against human BIRC5 (aa 1-142) recombinant protein. (Expected MW is 16 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) .
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MSCs cells using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cervical cancer (upper) and human colon cancer (bottom) tissues using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) (green) and negative control (red) .
- Validation comment
- Flow Cytometry
