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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA501245 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501245, RRID:AB_11130006
- Product name
- Anti-BIRC5/Survivin mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
- Antibody type
- Monoclonal
- Description
- Anti-BIRC5/Survivin mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BIRC5
- Isotype
- IgG
- Antibody clone number
- OTI2D9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Human Parvovirus Infection of Human Airway Epithelia Induces Pyroptotic Cell Death by Inhibiting Apoptosis.
Deng X, Zou W, Xiong M, Wang Z, Engelhardt JF, Ye SQ, Yan Z, Qiu J
Journal of virology 2017 Dec 15;91(24)
Journal of virology 2017 Dec 15;91(24)
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 .
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-BIRC5 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BIRC5 mouse monoclonal antibody (TA501245) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIRC5 (RC205935).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA501245, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA501245, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205935 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BIRC5 antibody(TA501245), and then analyzed by flow cytometry.
- Validation comment
- FC
