AF1008

antibody from Novus Biologicals

Targeting: LTBR D12S370, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFRSF3

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1008

Provider

Novus Biologicals

Product name

Goat Polyclonal Lymphotoxin beta R/TNFRSF3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Lymphotoxin beta R/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.

Marrero L, Simkin J, Sammarco M, Muneoka K
Regeneration (Oxford, England) 2017 Apr;4(2):69-84

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A dendritic-cell-stromal axis maintains immune responses in lymph nodes.

Kumar V, Dasoveanu DC, Chyou S, Tzeng TC, Rozo C, Liang Y, Stohl W, Fu YX, Ruddle NH, Lu TT
Immunity 2015 Apr 21;42(4):719-30

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Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.

Katakai T, Kondo N, Ueda Y, Kinashi T
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):617-26

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TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.

Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
The Journal of clinical investigation 2009 Oct;119(10):2954-64

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CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways.

Suto H, Katakai T, Sugai M, Kinashi T, Shimizu A
International immunology 2009 Apr;21(4):467-76

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Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.

Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6189-200

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Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.

Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6189-200

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Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes.

Katakai T, Hara T, Sugai M, Gonda H, Shimizu A
The Journal of experimental medicine 2004 Sep 20;200(6):783-95

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Lymphotoxin beta R/TNFRSF3 in NIH-3T3 Mouse Cell Line by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.