PA5-21920

antibody from Invitrogen Antibodies

Targeting: COX6B1 COX6B, COXG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21920

Provider

Invitrogen Antibodies

Product name

COX6B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, HeLa, HepG2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of COX6B1 using 30 µg of HepG2 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a COX6B1 polyclonal antibody (Product # PA5-21920) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using COX6B1 Polyclonal Antibody (Product # PA5-21920). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX6B1 Polyclonal Antibody (Product # PA5-21920) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of COX6B1 showing staining in the mitochondria of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a COX6B1 polyclonal antibody (Product # PA5-21920) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COX6B1 Polyclonal Antibody detects COX6B1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COX6B1 protein stained by COX6B1 Polyclonal Antibody (Product # PA5-21920) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded BCC3 xenograft, using COX6B1 (Product # PA5-21920) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.