PA5-88906

antibody from Invitrogen Antibodies

Targeting: RPL32 L32

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88906

Provider

Invitrogen Antibodies

Product name

RPL32 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI RVTNPNARLR SEENE; Positive Samples: A-549, HepG2, NIH/3T3, Mouse liver, Mouse stomach, Mouse spleen, Rat ovary

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.44 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using RPL32 Polyclonal antibody (Product # PA5-88906) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RPL32 in extracts of various cell lines using RPL32 Polyclonal Antibody (Product # PA5-88906) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPL32 was performed in L929 cells using RPL32 Polyclonal Antibody (Product # PA5-88906).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPL32 was performed in L929 cells using RPL32 Polyclonal Antibody (Product # PA5-88906).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPL32 was performed in U-2 OS cells using RPL32 Polyclonal Antibody (Product # PA5-88906).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL32 in paraffin-embedded rat brain using RPL32 Polyclonal Antibody (Product # PA5-88906) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL32 in paraffin-embedded human lung cancer using RPL32 Polyclonal Antibody (Product # PA5-88906) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL32 in paraffin-embedded mouse spinal cord using RPL32 Polyclonal Antibody (Product # PA5-88906) at a dilution of 1:100.