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- Validations
- Western blot [2]
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- Product number
- PA5-50914 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta-3 Adrenergic Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- ADRB3 Polyclonal Antibody detects endogenous levels of ADRB3 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages.
Miller CN, Samuels JS, Azhar Y, Parmar A, Shashidharamurthy R, Rayalam S
Medicines (Basel, Switzerland) 2019 Jan 31;6(1)
Medicines (Basel, Switzerland) 2019 Jan 31;6(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADRB3 in K562 cells. Samples were probed using ADRB3 Polyclonal Antibody (Product # PA5-50914).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-beta-3 Adrenergic Receptor Polyclonal Antibody (Product # PA5-50914) and a 75 kDa band corresponding to beta-3 Adrenergic Receptor was observed in 3T3-L1 differentiated to adipocytes and Mouse Brown Adipose tissue with little or no expression in 3T3-L1, Mouse Adipose tissue, Mouse Liver, Rat Liver and Mouse Brain which are reported negative for beta-3 Adrenergic Receptor expression. Whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to Adipocytes (Lane 2) and tissue extracts (30 µg lysate) of Mouse Brown Adipose tissue (Lane 3), Mouse Adipose tissue (Lane 4), Mouse Liver (Lane 5), Rat Liver (Lane 6) and Mouse Brain (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
