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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1597T-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fc gamma RIIIB/CD16b Alexa Fluor® 594-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Fc gamma RIIIB/CD16b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Fc gamma RIIA or recombinant mouse Fc gamma RIII is observed. In flow cytometry of whole blood, recognition of Fc gamma RIIIB on the granulocyte population, but not Fc gamma RIIIA on NK cells, is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
O75015- Isotype
- IgG
- Antibody clone number
- 245514
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Fc gamma RIIIB/CD16b in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Fc gamma RIIIB/CD16)b Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB1597T, filled histogram) or Mouse IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC0041T, open histogram). View our protocol for Staining Membrane-associated Proteins.
