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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- TA503121 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503121, RRID:AB_11126784
- Product name
- LSM1 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)
- Antibody type
- Monoclonal
- Description
- LSM1 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LSM1
- Isotype
- IgG
- Antibody clone number
- OTI5E6
- Vial size
- 100 µl
- Concentration
- 0.57 mg/ml
Submitted references Reactivation of nonsense-mediated mRNA decay protects against C9orf72 dipeptide-repeat neurotoxicity.
Xu W, Bao P, Jiang X, Wang H, Qin M, Wang R, Wang T, Yang Y, Lorenzini I, Liao L, Sattler R, Xu J
Brain : a journal of neurology 2019 May 1;142(5):1349-1364
Brain : a journal of neurology 2019 May 1;142(5):1349-1364
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LSM1 (RC200288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LSM1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LSM1 mouse monoclonal antibody (TA503121) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LSM1(RC200288).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200288 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LSM1 antibody(TA503121), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-LSM1 antibody(TA503121),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
