Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAGLN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, A549, HeLa, mouse brain.
- Concentration
- 0.53 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Transgelin 2 using 30 µg of A) A549 and B) HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Transgelin 2 polyclonal antibody (Product # PA5-30271) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TAGLN2 Polyclonal Antibody (Product # PA5-30271). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. TAGLN2 Polyclonal Antibody (Product # PA5-30271) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transgelin 2 antibody detects Transgelin 2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody TAGLN2 Polyclonal Antibody (Product # PA5-30271) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TAGLN2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN2 Polyclonal Antibody (Product # PA5-30271) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TAGLN2 Polyclonal Antibody detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Transgelin 2 protein stained by TAGLN2 Polyclonal Antibody (Product # PA5-30271) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN2 was performed in paraffin-embedded mouse prostate tissue using TAGLN2 Polyclonal Antibody (Product # PA5-30271) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TAGLN2 was performed in paraffin-embedded mouse pancreas tissue using TAGLN2 Polyclonal Antibody (Product # PA5-30271) at a dilution of 1:500.
