Antibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Flow cytometry [1]
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- Product number
- 14-1038-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: The B-Ly7 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B-Ly7
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Inflammatory cues enhance TGFβ activation by distinct subsets of human intestinal dendritic cells via integrin αvβ8.
Optimization of mass cytometry sample cryopreservation after staining.
Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection.
RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.
Rate of AIDS progression is associated with gastrointestinal dysfunction in simian immunodeficiency virus-infected pigtail macaques.
DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues.
Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.
Immunogold labeling for the diagnosis of leukemia by transmission and scanning electron microscopy.
Immunogold labelling of leukemic hairy cells with the B-ly7 monoclonal antibody: an SEM and TEM study.
Reactivity of monoclonal antibody B-ly7 with a subset of activated T cells and T-cell lymphomas.
Monoclonal antibody B-ly7: a sensitive marker for detection of minimal residual disease in hairy cell leukemia.
Specificities of monoclonal antibodies B-ly7 and HML-1 are identical.
Fenton TM, Kelly A, Shuttleworth EE, Smedley C, Atakilit A, Powrie F, Campbell S, Nishimura SL, Sheppard D, Levison S, Worthington JJ, Lehtinen MJ, Travis MA
Mucosal immunology 2017 May;10(3):624-634
Mucosal immunology 2017 May;10(3):624-634
Optimization of mass cytometry sample cryopreservation after staining.
Sumatoh HR, Teng KW, Cheng Y, Newell EW
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61
Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection.
Pichyangkul S, Yongvanitchit K, Limsalakpetch A, Kum-Arb U, Im-Erbsin R, Boonnak K, Thitithayanont A, Jongkaewwattana A, Wiboon-ut S, Mongkolsirichaikul D, Mahanonda R, Spring M, Chuang I, Mason CJ, Saunders DL
Journal of immunology (Baltimore, Md. : 1950) 2015 Nov 1;195(9):4378-86
Journal of immunology (Baltimore, Md. : 1950) 2015 Nov 1;195(9):4378-86
RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.
Jozwik A, Habibi MS, Paras A, Zhu J, Guvenel A, Dhariwal J, Almond M, Wong EHC, Sykes A, Maybeno M, Del Rosario J, Trujillo-Torralbo MB, Mallia P, Sidney J, Peters B, Kon OM, Sette A, Johnston SL, Openshaw PJ, Chiu C
Nature communications 2015 Dec 21;6:10224
Nature communications 2015 Dec 21;6:10224
Rate of AIDS progression is associated with gastrointestinal dysfunction in simian immunodeficiency virus-infected pigtail macaques.
Canary LA, Vinton CL, Morcock DR, Pierce JB, Estes JD, Brenchley JM, Klatt NR
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2959-65
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2959-65
DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues.
Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, Håkansson UK, Moita LF, Agace WW, Bonnet D, Reis e Sousa C
Blood 2012 Jun 21;119(25):6052-62
Blood 2012 Jun 21;119(25):6052-62
Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.
Poulin LF, Salio M, Griessinger E, Anjos-Afonso F, Craciun L, Chen JL, Keller AM, Joffre O, Zelenay S, Nye E, Le Moine A, Faure F, Donckier V, Sancho D, Cerundolo V, Bonnet D, Reis e Sousa C
The Journal of experimental medicine 2010 Jun 7;207(6):1261-71
The Journal of experimental medicine 2010 Jun 7;207(6):1261-71
Immunogold labeling for the diagnosis of leukemia by transmission and scanning electron microscopy.
de Harven E, Soligo D, Christensen H
Scanning microscopy 1995;9(4):1191-199; discussion 1199-1201
Scanning microscopy 1995;9(4):1191-199; discussion 1199-1201
Immunogold labelling of leukemic hairy cells with the B-ly7 monoclonal antibody: an SEM and TEM study.
de Harven E, Christensen H, Poppema S, Scott JG
Microscopy research and technique 1994 Jul 1;28(4):356-67
Microscopy research and technique 1994 Jul 1;28(4):356-67
Reactivity of monoclonal antibody B-ly7 with a subset of activated T cells and T-cell lymphomas.
Visser L, Dabbagh L, Poppema S
Hematologic pathology 1992;6(1):37-42
Hematologic pathology 1992;6(1):37-42
Monoclonal antibody B-ly7: a sensitive marker for detection of minimal residual disease in hairy cell leukemia.
Thaler J, Dietze O, Faber V, Greil R, Gastl G, Denz H, Ho AD, Huber H
Leukemia 1990 Mar;4(3):170-6
Leukemia 1990 Mar;4(3):170-6
Specificities of monoclonal antibodies B-ly7 and HML-1 are identical.
Schwarting R, Dienemann D, Kruschwitz M, Fritsche G, Stein H
Blood 1990 Jan 1;75(1):320-1
Blood 1990 Jan 1;75(1):320-1
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.