370506

GZMK antibody from BioLegend

PRSS, TRYP2

Immunocytochemistry

Data presented on provider website

Other assay

Supportive data in Antibodypedia

Provider product page for 370506

Antibody Data

Product number

370506

Provider

BioLegend

Proper citation

BioLegend Cat#370506, RRID:AB_2629600

Product name

Alexa Fluor® 594 anti-human Granzyme K

Provider product page

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

GM26E7

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood using a Ficol Gradient method. The PBMCs were washed and fixed with 2% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 0.5% FBS for 30 minutes. After blocking, the cells were incubated with Granzyme K (clone GM26E7) Alexa Fluor® 594 (red) and CD8 (clone HIT8a) Alexa Fluor® 488 (green) for 30 minutes at room temperature. The cells were spun onto a microscopy slide with Cytospin. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.

Conjugate

Yellow dye