Antibody data

Product number
46-8897-42
Provider
Invitrogen Antibodies
Product name
Anti-Granzyme K Monoclonal Antibody (G3H69), PerCP-eFluor 710, eBioscience™
Provider product page
Invitrogen Antibodies - 46-8897-42
Antibody type
Monoclonal
Antigen
Other
Description
Description: This G3H69 monoclonal antibody reacts with human Granzyme K. Granzyme K is one of five granzyme serine proteases that have been identified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Granzyme A and Granzyme K are both tryptases and appear to show overlapping, though not identical, substrate specificity. This functional similarity is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs. Applications Reported: This G3H69 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This G3H69 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood monocytes. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
G3H69
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
Provider Type Product Number
- No reagents -