F40407

antibody from NSJ Bioreagents

Targeting: PINK1 BRPK, PARK6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F40407

Provider

NSJ Bioreagents

Product name

PINK1 Antibody

Antibody type

Monoclonal

Antigen

Recombinant protein was used to produced this monoclonal PINK1 antibody.

Description

Purified antibody

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

38CT20.8.5

Vial size

80 µl, 400 µl

Storage

Aliquot the PINK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) A431 cell line and (2) mouse brain lysate using Pink1 antibody at 1:1000. Predicted molecular weight: 60-70 kDa

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of PINK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene. Predicted molecular weight: 60-70 kDa

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of human 1) A431, 2) HeLa, 3) MCF7, 4) 293T and 5) mouse brain lysate with PINK1 antibody. Predicted molecular weight: 60-70 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Fluorescent image of PC12 cells stained with Pink1 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488 secondary was used (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human heart using Pink1 antibody at 1:25 dilution.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human stomach using Pink1 antibody at 1:25 dilution.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

PINK1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma.