PA5-29056

antibody from Invitrogen Antibodies

Targeting: MARS1 CMT2U, MARS, MetRS, SPG70

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29056

Provider

Invitrogen Antibodies

Product name

MARS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain.

Concentration

0.67 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MARS using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MARS polyclonal antibody (Product # PA5-29056) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MARS was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MARS Polyclonal Antibody (Product # PA5-29056) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MARS was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MARS Polyclonal Antibody (Product # PA5-29056) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MARS was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: MARS Polyclonal Antibody (Product # PA5-29056) diluted at 1:500. Blue: Hoechst 33342 staining.