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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- R31556 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Bub1 Antibody
- Antibody type
- Polyclonal
- Antigen
- Human partial recombinant protein (AA 731-1085) was used as the immunogen for this Bub1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Bub1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Bub1 antibody and Lane 1: rat testis; 2: (r) ovary; 3: (r) liver; 4: human Jurkat; 5: (h) COLO320; 6: (h) HEPG2. Expected/observed size ~122KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Bub1 antibody and recombinant human protein (0.5ng)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) HeLa, 3) 293T, 4) A431, 5) Caco-2, 6) PC-3, 7) MCF7, 8) U-2 OS, 9) rat testis and 10) mouse testis lysate with Bub1 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa.
