AP12772A

antibody from Abgent

Targeting: RPL15 EC45, L15, RPL10, RPLY10, RPYL10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP12772A

Provider

Abgent

Proper citation

Abgent Cat#AP12772a, RRID:AB_10817414

Product name

RPL15 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPL15 Antibody (N-term) (Cat. #AP12772a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RPL15 antibody detected the RPL15 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPL15 Antibody (N-term) (Cat. #AP12772a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RPL15 antibody detected the RPL15 protein (arrow).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPL15 Antibody (N-term) (Cat. #AP12772a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RPL15 Antibody (N-term) (Cat. #AP12772a) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50