Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-96958 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAATASAGAG GIDGKPRTSP KSVKFLFGGL AGMGATVFVQ PLDLVKNRMQ LSGEGAKTRE YKTSFHALTS ILKAEGLRGI YTGLSAGLLR QATYTTTRLG IYTVLFERLT GADGTPPGFL LKAVIGMTAG ATGAFVGTPA EVALIRMTAD GRLPADQRRG YKNVFNALIR ITREEGVLTL WRGCIPTMAR AVVVNAAQLA SYSQSKQFLL DSGYFSDNIL CHFCASMISG LVTTAASMPV DIAKTRIQNM RMIDGKPEYK NGLDVLFKVV RYEGFFSLWK GFTPYYARLG PHTVLTFIFL EQMNKAYKRL FLSG; Positive Samples: MCF7, Raji, H460, Mouse brain, Rat brain; Cellular Location: Mitochondrion inner membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.09 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SLC25A11 Polyclonal antibody (Product # PA5-96958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC25A11 in extracts of various cell lines using SLC25A11 Polyclonal Antibody (Product # PA5-96958) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A11 in paraffin-embedded rat spleen using SLC25A11 Polyclonal Antibody (Product # PA5-96958) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A11 in paraffin-embedded human colon carcinoma using SLC25A11 Polyclonal Antibody (Product # PA5-96958) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A11 in paraffin-embedded human gastric cancer using SLC25A11 Polyclonal Antibody (Product # PA5-96958) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC25A11 in paraffin-embedded mouse spinal cord using SLC25A11 Polyclonal Antibody (Product # PA5-96958) at a dilution of 1:100.