PA5-27510

antibody from Invitrogen Antibodies

Targeting: SLC25A11 OGC, SLC20A4

Antibody data

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Validation data

SLC25A11 protein structure - PA5-27510 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SLC25A11 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLC25A11 Polyclonal Antibody (Product # PA5-27510) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SLC25A11 Polyclonal Antibody (Product # PA5-27510). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: Raji. 12% SDS PAGE. SLC25A11 Polyclonal Antibody (Product # PA5-27510) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using SLC25A11 Polyclonal Antibody (Product # PA5-27510) and a 30 kDa band corresponding to SLC25A11 was observed across cell lines. Whole cell extracts (40 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3), Mouse heart (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).