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Learn600-401-934
antibody from Rockland Immunochemicals, Inc.
Targeting: EIF3F
eIF3-epsilon, eIF3-p47, EIF3S5
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- 600-401-934 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-934, RRID:AB_2096906
- Product name
- Anti-eIF3f (RABBIT) Antibody - 600-401-934
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references A cyclin-dependent kinase, CDK11/p58, represses cap-dependent translation during mitosis.
A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling.
Glycine restores the anabolic response to leucine in a mouse model of acute inflammation.
An S, Kwon OS, Yu J, Jang SK
Cellular and molecular life sciences : CMLS 2020 Nov;77(22):4693-4708
Cellular and molecular life sciences : CMLS 2020 Nov;77(22):4693-4708
A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling.
You JS, Dooley MS, Kim CR, Kim EJ, Xu W, Goodman CA, Hornberger TA
Science signaling 2018 May 15;11(530)
Science signaling 2018 May 15;11(530)
Glycine restores the anabolic response to leucine in a mouse model of acute inflammation.
Ham DJ, Caldow MK, Chhen V, Chee A, Wang X, Proud CG, Lynch GS, Koopman R
American journal of physiology. Endocrinology and metabolism 2016 Jun 1;310(11):E970-81
American journal of physiology. Endocrinology and metabolism 2016 Jun 1;310(11):E970-81
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.
- Validation comment
- Western Blot
