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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00007327-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007327-M01, RRID:AB_2210452
- Product name
- Mouse Monoclonal UBE2G2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. UBE2G2 - ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Ataxin-3 deubiquitination is coupled to Parkin ubiquitination via E2 ubiquitin-conjugating enzyme.
Durcan TM, Kontogiannea M, Bedard N, Wing SS, Fon EA
The Journal of biological chemistry 2012 Jan 2;287(1):531-41
The Journal of biological chemistry 2012 Jan 2;287(1):531-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2G2 Antibody (5E1) [H00007327-M01] - Analysis of UBE2G2 expression in transfected 293T cell line by UBE2G2 monoclonal antibody (M01), clone 5E1.Lane 1: UBE2G2 transfected lysate(15.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2G2 Antibody (5E1) [H00007327-M01] - UBE2G2 monoclonal antibody (M01), clone 5E1 Analysis of UBE2G2 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: UBE2G2 Antibody (5E1) [H00007327-M01] - Detection limit for recombinant GST tagged UBE2G2 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBE2G2 Antibody (5E1) [H00007327-M01] - Analysis of monoclonal antibody to UBE2G2 on formalin-fixed paraffin-embedded human liver. Antibody concentration 1 ug/ml.
