LS-C818241

antibody from LSBio

Targeting: PPM1A MGC9201, PP2CA, PP2Calpha

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C818241

Provider

LSBio

Product name

PPM1A / PP2CA Antibody (Preservative Free) LS-C818241

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

PPM1A was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. 4 uL anti-PPM1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PPM1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

PPM1A was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. 4 uL anti-PPM1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PPM1A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.