H00007328-M01

antibody from Abnova Corporation

Targeting: UBE2H GID3, UBC8, UBCH

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007328-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007328-M01, RRID:AB_425732

Product name

UBE2H monoclonal antibody (M01), clone 3C4-1A2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant UBE2H.

Antigen sequence

MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFV
VKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGF
MNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIF
ESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQK
IKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSE
DEAQDMEL


Isotype

IgG

Antibody clone number

3C4-1A2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

UBE2H monoclonal antibody (M01), clone 3C4-1A2 Western Blot analysis of UBE2H expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody (M01), clone 3C4-1A2.Lane 1: UBE2H transfected lysate(20.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UBE2H is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol