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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-112141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product can be used in direct ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UBE2H using UBE2H Polyclonal Antibody (Product # PA5-112141) at a dilution of 1:500. Lane A: Hela whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa (We are unsure as to the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of UBE2H was performed on (Lane A) 0.5 mg Hela whole cell lysate using 2 µL of UBE2H Polyclonal Antibody (Product # PA5-112141) at a dilution of 200, and 15 µL of 50 % Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) was used as a secondary antibody at a dilution of 1:5000. Developed using the odssey technique and performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa.
