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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [2]
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- Product number
- NB100-74604 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74604, RRID:AB_1049024
- Product name
- Rabbit Polyclonal JMJD2B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Histone demethylase KDM4B regulates otic vesicle invagination via epigenetic control of Dlx3 expression.
Uribe RA, Buzzi AL, Bronner ME, Strobl-Mazzulla PH
The Journal of cell biology 2015 Nov 23;211(4):815-27
The Journal of cell biology 2015 Nov 23;211(4):815-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: JMJD2B Antibody [NB100-74604] - Detection of Human JMJD2B by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3 mcg/mg lysate. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74605, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: JMJD2B Antibody [NB100-74604] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3 ug per reaction. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74605. For blotting immunoprecipitated JMJD2B, NB100-74604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
