AP52271PU-N

antibody from Acris Antibodies GmbH

Targeting: KDM4B JMJD2B, KIAA0876, TDRD14B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP52271PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP52271PU-N, RRID:AB_11142956

Product name

anti JMJD2B / KDM4B

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 287~316 amino acids from the Thr305 region of human JMJD2B

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.4 ml

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Phospho-JMJD2B-pT305 Cat.-No AP52271PU-N in mouse spleen tissue lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue reacted with JMJD2B-T305 Antibody Cat.-No AP52271PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells using JMJD2B-T305 Antibody Cat.-No AP52271PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.