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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
- Flow cytometry [2]
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Validation data
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- Product number
- AM26624AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti CD148 / PTPRJ
- Antibody type
- Monoclonal
- Antigen
- Human peripheral blood mononuclear cells
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- #255
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of CD148 from biotinylated PBMC with mouse IgG2a (1) or AM26624AF-N (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with HRP-Streptavidin
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CD148 expression on U937 cells. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26624AF-N to the cells.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CD148 expression on Lymphocyte (1), Monocyte (2), and Granulocyte (3). The staining intensity of AM26624AF-N is shown in the horizontal axis with CD11b-FITC staining on the vertical axis.
