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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324376 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal His6-DEP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal His6-DEP-1 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PTPRJ
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 1.5 mg/ml
Submitted references Comparative proteomics of a model MCF10A-KRasG12V cell line reveals a distinct molecular signature of the KRasG12V cell surface.
Ye X, Chan KC, Waters AM, Bess M, Harned A, Wei BR, Loncarek J, Luke BT, Orsburn BC, Hollinger BD, Stephens RM, Bagni R, Martinko A, Wells JA, Nissley DV, McCormick F, Whiteley G, Blonder J
Oncotarget 2016 Dec 27;7(52):86948-86971
Oncotarget 2016 Dec 27;7(52):86948-86971
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- His6-DEP-1 (Cat. #TA324376) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DEP-1 antibody detected the DEP-1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of His6-DEP-1 Antibody (Cat. #TA324376) with Hela cell followed by Alexa Fluor?????488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- His6-DEP-1 Antibody (Cat. #TA324376)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of His6-DEP-1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
