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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-2595 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2595, RRID:AB_2240386
- Product name
- Rabbit Polyclonal PIMT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PIMT Antibody [NB100-2595] - Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PIMT antibody NB100-2595 (lot NB100-2595-2) used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by a previous lot of this antibody (lot NB100-2595-1) and rabbit anti-PIMT antibody NBP2-44294. For blotting immunoprecipitated PIMT, NB100-2595 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-PIMT used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PIMT Antibody [NB100-2595] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:200 (1ug/ml). Detection: DAB
