Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-0949-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD94 Monoclonal Antibody (DX22), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The DX22 monoclonal antibody reacts with human CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells, a subset of gd T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage. Applications Reported: The DX22 antibody has been reported for use in flow cytometric analysis. Applications Tested: This DX22 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- DX22
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
Changes of cytokines during a spaceflight analog--a 45-day head-down bed rest.
Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.
Neuron-interacting satellite glial cells in human trigeminal ganglia have an APC phenotype.
Human natural killer cell receptors involved in MHC class I recognition are disulfide-linked heterodimers of CD94 and NKG2 subunits.
CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes.
Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily.
Cuff AO, Robertson FP, Stegmann KA, Pallett LJ, Maini MK, Davidson BR, Male V
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Changes of cytokines during a spaceflight analog--a 45-day head-down bed rest.
Xu X, Tan C, Li P, Zhang S, Pang X, Liu H, Li L, Sun X, Zhang Y, Wu H, Chen X, Ge Q
PloS one 2013;8(10):e77401
PloS one 2013;8(10):e77401
Liver-resident NK cells confer adaptive immunity in skin-contact inflammation.
Peng H, Jiang X, Chen Y, Sojka DK, Wei H, Gao X, Sun R, Yokoyama WM, Tian Z
The Journal of clinical investigation 2013 Apr;123(4):1444-56
The Journal of clinical investigation 2013 Apr;123(4):1444-56
Neuron-interacting satellite glial cells in human trigeminal ganglia have an APC phenotype.
van Velzen M, Laman JD, Kleinjan A, Poot A, Osterhaus AD, Verjans GM
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 15;183(4):2456-61
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 15;183(4):2456-61
Human natural killer cell receptors involved in MHC class I recognition are disulfide-linked heterodimers of CD94 and NKG2 subunits.
Lazetic S, Chang C, Houchins JP, Lanier LL, Phillips JH
Journal of immunology (Baltimore, Md. : 1950) 1996 Dec 1;157(11):4741-5
Journal of immunology (Baltimore, Md. : 1950) 1996 Dec 1;157(11):4741-5
CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes.
Phillips JH, Chang C, Mattson J, Gumperz JE, Parham P, Lanier LL
Immunity 1996 Aug;5(2):163-72
Immunity 1996 Aug;5(2):163-72
Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily.
Chang C, Rodríguez A, Carretero M, López-Botet M, Phillips JH, Lanier LL
European journal of immunology 1995 Sep;25(9):2433-7
European journal of immunology 1995 Sep;25(9):2433-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (dotted histogram) Anti-Human CD94 FITC (black histogram).
- Conjugate
- Green dye