Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1058 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Canine CD94 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects recombinant human CD94 in direct ELISAs and human, mouse, and canine CD94 in Western blots.
- Reactivity
- Human, Mouse, Canine
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q13241- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Canine CD94 by Western Blot. Western blot shows lysates of CLL-1390 canine leukocytic round cell neoplasia cell line, HDLM human Hodgkin’s lymphoma cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD94 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD94 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
