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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX80649 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX80649, RRID:AB_626056
- Product name
- AKAP12 antibody
- Antibody type
- Polyclonal
- Reactivity
- Rat
- Host
- Sheep
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The membrane was probed with 5 ?g/ml of pre-immune serum or 5 ?g of SSecks antibody (3 second exposure shown). The blot was then washed and probed with monoclonal anti-sheep IgG antibody coupled to horseradish peroxidase 1:10,000, and detected with luminol.
