Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA3-020 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FKBP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-020 detects heat shock protein 56 (HSP56), also known as FKBP4/FKBP52, from human, monkey, mouse and rabbit tissues.
- Concentration
- 1 mg/mL
Submitted references FK506-binding protein 52 is essential to uterine reproductive physiology controlled by the progesterone receptor A isoform.
Protection against ischemic brain damage in rats by immunophilin ligand GPI-1046.
Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.
Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.
Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex.
Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.
Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.
Yang Z, Wolf IM, Chen H, Periyasamy S, Chen Z, Yong W, Shi S, Zhao W, Xu J, Srivastava A, Sánchez ER, Shou W
Molecular endocrinology (Baltimore, Md.) 2006 Nov;20(11):2682-94
Molecular endocrinology (Baltimore, Md.) 2006 Nov;20(11):2682-94
Protection against ischemic brain damage in rats by immunophilin ligand GPI-1046.
Li F, Omori N, Hayashi T, Jin G, Sato K, Nagano I, Shoji M, Abe K
Journal of neuroscience research 2004 May 1;76(3):383-9
Journal of neuroscience research 2004 May 1;76(3):383-9
Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.
Manabe Y, Warita H, Murakami T, Shiote M, Hayashi T, Omori N, Nagano I, Shoji M, Abe K
Brain research 2002 May 10;935(1-2):124-8
Brain research 2002 May 10;935(1-2):124-8
Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.
Manabe Y, Warita H, Murakami T, Shiote M, Hayashi T, Omori N, Nagano I, Shoji M, Abe K
Brain research 2002 May 10;935(1-2):124-8
Brain research 2002 May 10;935(1-2):124-8
Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex.
Uittenbogaard A, Everson WV, Matveev SV, Smart EJ
The Journal of biological chemistry 2002 Feb 15;277(7):4925-31
The Journal of biological chemistry 2002 Feb 15;277(7):4925-31
Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.
Smith DF, Whitesell L, Nair SC, Chen S, Prapapanich V, Rimerman RA
Molecular and cellular biology 1995 Dec;15(12):6804-12
Molecular and cellular biology 1995 Dec;15(12):6804-12
Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.
Smith DF, Whitesell L, Nair SC, Chen S, Prapapanich V, Rimerman RA
Molecular and cellular biology 1995 Dec;15(12):6804-12
Molecular and cellular biology 1995 Dec;15(12):6804-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of Hsp56 on human cell line S49 cytosol using Product # PA3-020.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Heat Shock Protein 56 (HSP56) was performed by loading 50 µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Heat Shock protein 56 (HSP56, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL