PAB20805

antibody from Abnova Corporation

Targeting: GPRC5B RAIG-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20805

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20805, RRID:AB_10966940

Product name

GPRC5B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GPRC5B.

Antigen sequence

IHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQL
PRAYMENKAFSMDEHNAALRTAGFPNGSLGKRPSG
SLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with GPRC5B polyclonal antibody (Cat # PAB20805) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with GPRC5B polyclonal antibody (Cat # PAB20805) at 1-4 ug/mL dilution shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with GPRC5B polyclonal antibody (Cat # PAB20805) shows membranous positivity in glomeruli at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)