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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-28747 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GALR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse eye, mouse brain, rat brain.
- Concentration
- 0.72 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GALR3 using A) 30 µg HeLa whole cell lysate and B) 30 µg Raji whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GALR3 polyclonal antibody (Product # PA5-28747) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GALR3 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GALR3 Polyclonal Antibody (Product # PA5-28747) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GALR3 Polyclonal Antibody (Product # PA5-28747). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GALR3 Polyclonal Antibody (Product # PA5-28747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GALR3 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GALR3 Polyclonal Antibody (Product # PA5-28747) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using GALR3 (Product # PA5-28747) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
