AM32709PU-N

antibody from Acris Antibodies GmbH

Targeting: MYOD1 bHLHc1, MYF3, MYOD, PUM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM32709PU-N

Provider

Acris Antibodies GmbH

Product name

anti MYOD1

Antibody type

Monoclonal

Antigen

14 amino acid peptide whose sequence corresponds to a region that is specific to the MyoD protein and is distal (away) from the homologous helix-loop-helix region of the MyoD protein that is shared by other members of the myogenic bHLH transcription factors.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

BC1-C2H8

Vial size

0.1 mg

Concentration

0.5 mg/ml (Lot specific)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blotting with MyoD Antibody Cat.-No AM32709PU-N (Clone BC1-C2H8) used at 2, 3 and 5 µg/ml (in Blotto) on Rh30 rhabdomyosarcoma whole cell lysate and nuclear extract loaded at 10 µg/lane. W= Whole cell lysate, N= Nuclear extract.Secondary Antibody: Peroxidase conjugated Rabbit anti-Mouse Ig used at 1/10,000 dilution in Blotto. Developed by enhanced chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Staining of Rh30 rhabdomyosarcoma cells at using MyoD Antibody Cat.-No AM32709PU-N (Clone BC1-C2H8) at 5 µg/ml is shown. Secondary Antibody: FITC conjugated anti Mouse Ig.