MA5-24292

antibody from Invitrogen Antibodies

Targeting: PRDM14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24292

Provider

Invitrogen Antibodies

Product name

PRDM14 Monoclonal Antibody (647002)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant mouse PRDM14, recombinant human (rh) ZNF24, rhZNF143, rhZNF206 or rhZNF281 is observed.

Antibody clone number

647002

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of human PRDM14 Monoclonal Antibody (Product # MA5-24292) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for PRDM14 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRDM14 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in PRDM14 monoclonal antibody (Product # MA5-24292) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for PRDM14 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PRDM14 in fixed BG01V human embryonic stem cells grown on irradiated MEF cells. Samples were incubated in PRDM14 monoclonal antibody (Product # MA5-24292) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PRDM14 in fixed BG01V human embryonic stem cells grown on irradiated MEF cells. Samples were incubated in PRDM14 monoclonal antibody (Product # MA5-24292) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.